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Ivyo bihimba vy’umubiri bita Ban-Lan-Gen bigabanya indwara y’umusonga idakira iterwa n’ivyo bita dextran sodium sulfate mu mbeba biciye mu guhindura imigera yo mu mara no kugarura SCFA Derived-GLP-1 yo mu mara
Igisata c’ubuvuzi c’abantu co muri kaminuza ya Peking, i Shenzhen, mu gisagara ca Shenzhen; 2Ishure ry’ubuhinga bw’ubuzima ryo muri kaminuza ya Shenzhen, i Shenzhen, muri Repubulika y’Ubushinwa; 3Guizhou Medical University Ikigo c’ubushakashatsi c’ubuhinga bwa none c’ubuvuzi bw’ubwoko n’ubuvuzi bwa kera bw’abashinwa Iterambere n’ugukoresha Minisiteri y’uburezi, Laboratwari y’ingenzi y’ubuvuzi bw’intara ya Guizhou, Kaminuza y’ubuvuzi ya Guizhou , Guiyang, Repubulika y’Ubushinwa; 4 Igisata c’ubuvuzi bw’indwara z’ibihaha, mu bitaro vya kaminuza ya Peking Shenzhen, i Shenzhen, muri Repubulika y’Ubushinwa; 5 Ishure ry’ubuvuzi, Kaminuza y’ubuvuzi ya Guizhou, Laboratwari y’Igihugu y’Ibikorwa n’Ikoreshwa ry’Ibimera vy’Ubuvuzi, Guiyang; 6 Igisata c’ubuvuzi bwo mu laboratwari, Kaminuza ya Peking Ibitaro vya Shenzhen, Shenzhen, Ubushinwa [email irindwa] Shen Xiangchun, Ishure ry’ubuvuzi, Kaminuza y’ubuvuzi ya Guizhou, Guizhou, Repubulika y’Ubushinwa, 550004, Email [email irindwa] Intumbero: ubuvuzi bushasha bushingiye kuri GLP-1 ni uburyo bushasha bwo kuvura umururumba indwara.Ban-Lan-Gen (BLG) granules ni umuti uzwi cane wo kurwanya umugera wa TCM ugaragaza ubushobozi bwo kurwanya umururumba mu kuvura indwara zitandukanye zitera umururumba.Ariko rero, ingaruka zawo zo kurwanya umururumba ku ndwara y’umusonga n’uburyo ukora ntibiramenyekana neza. (DSS)-induced chronic relapsing colitis in mice.Ibimenyetso vy’ibikorwa vy’ingwara, ibimenyetso vy’ibikomere, n’ingero za cytokine zitera umururumba vyakozwe kugira ngo hasuzumwe ingaruka z’uburinzi za BLG. gut microbiota composition, ingero za SCFAs z’amavyi, n’ugusohoka kwa GLP-1 mu turemangingo tw’imbeba tw’intango SCFA-derived GLP-1 production.Ivyavuyemwo: Ubuvuzi bwa BLG bwagabanuye cane ukubura ibiro, DAI, uguca bugufi kw’amaraso, kwonona utugingo ngengabuzima tw’amaraso, n’urugero rwa IL-βs cytokine y’amaraso, n’urugero rwa IL-βs rwo gutera umururumba. tissue.Ikindi, ubuvuzi bwa BLG burashobora gusubizaho cane Gcg, GPR41 na GRP43 mu maraso n’urugero rwa GLP-1 mu maraso y’imbeba zifise indwara y’ibihaha, no mu kwongerera imigera ivyara SCFA nka Akkermansia na Prevotellaceae_UCG-001, no kugabanya ubwinshi bw’imigera nk’uko Itsinda ry'imigera y'ibikoko, ibikoko vy'ibikoko vy'ibikoko vy'ibikoko vy'ibikoko vy'ibikoko vy'ibikoko vy'ibikoko vy'ibikoko vy'ibikoko vy'ibikoko vy'ibikoko. Ikindi, ubuvuzi bwa BLG burashobora kwongerera cane urugero rwa SCFAs mu mvyimba z’imbeba zifise indwara ya colitis. Muri ico gihe nyene, ivyigwa vyakozwe mu bipimo vy’ibikoko vyerekanye kandi ko amavyi y’imbeba zavuwe na BLG ashobora gutuma utugingo ngengabuzima dutoduto tw’imbere mu mbeba dusohora GLP-1. Insozero: Ivyo bimenyetso vyerekana ko BLG ifise ubushobozi bwo kurwanya indwara y’ibihaha. BLG irafise ubushobozi bwo gutegurwa nk’ubuvuzi, n’imiburiburi mu gice kimwe biciye mu guhindura imigera yo mu mara no kugarukana ivyimburwa vya GLP-1 biva kuri SCFA Imiti itanga icizigiro ku ndwara y’umusonga idakira. Amajambo nyamukuru: indwara y’umutima, utugingo ngengabuzima twa Ban-Lan-Gen, imigera y’amaraso, aside z’ibinure zifise urudodo rugufi, GLP-1.
Ulcerative colitis (UC) ni indwara y’umururumba w’igihe kirekire y’amaraso n’amaraso y’umubiri irangwa n’ugusubira gucibwamwo, ububabare bwo mu nda, kugabanya ibiro, n’amavyi y’amaraso.1 Vuba aha, ukwiragira kwa UC kwariko kuragwira mu bihugu vyari bifise abantu benshi cane, harimwo n’ibihugu vyo mu Burengero, mu Bushinwa. Ukwo kwiyongera gutera ingorane zikomeye ku buzima bwa bose kandi kugira ingaruka zikomeye ku bushobozi bw’abarwayi bwo gukora no ku buzima bwiza. ku UC, kandi intumbero y’ubuvuzi ni ugucungera ibimenyetso vy’ubuvuzi, gutuma umuntu agira ububabare, gutuma umubiri ukira, no kugabanya ukugaruka. Vuba aha, inyigisho nyinshi zerekanye ko ubuvuzi bwa kera bw’Abashinwa (TCM) bwagaragaje ubushobozi bukomeye bwo gufasha gukuraho UC n’ubumara buke, ivyo bikaba vyerekana ko gutegura ubuvuzi bushasha bwa TCM ari uburyo bwiza bwo kuvura UC.5-7.
Banlangen Granules (BLG) ni umuti w’abashinwa uteguwe mu mazi akuwe mu muzi wa Banlangen.8 Uretse ubushobozi bwawo bwo kurwanya imigera, BLG yerekana ubushobozi bwo kurwanya umururumba mu kuvura indwara zitandukanye zitera umururumba. glucoside zatanzwe kandi zimenyekana zivuye mu bihimba vy’amazi vy’igiti citwa Radix isatidis) n’ibindi bihimba vy’umubiri (hypoxanthine, adenosine, uridine na guanosine) be n’ibindi bihimba vy’umubiri nk’ivy’umubiri w’umuntu n’ivy’umubiri. colitis.13-17 Ariko rero, nta nyigisho zishingiye ku bimenyamenya zakozwe kugira ngo hasuzumwe ubushobozi bwa BLG mu ndwara y’ibihaha.Muri iki cigwa, twarondeye ingaruka z’uburinzi za BLG ku ndwara y’ibihaha idakira iterwa na dextran sodium sulfate (DSS) mu mbeba zafashwe C57BL/6 zihawe DSSi n’ugusubira inyuma kw’amaraso mu mbeba Umururumba, uburyo bwawo bwo kuwutunganya bujanye n’uguhindura imigera y’amaraso no gusubizaho ivyimburwa vy’amaraso bimeze nk’ivya glucagon-1 (GLP-1).
Ivyatsi vya BLG (bitagira isukari, vyemejwe na NMPA Z11020357; Ishirahamwe ry’Iterambere ry’Ikoranabuhanga ry’i Beijing, mu Bushinwa; inomero y’umugwi: 20110966) vyaguzwe mu ma farmasi. Louis, MO, USA). na aside ya butirike vyaguzwe mu nganda za Aladdin (Shanghai, mu Bushinwa).2-Aside ya butirike yaguzwe mu nganda za Merck KGaA (Darmstadt, mu Budagi).
Imbeba z’ingabo C57BL/6 zifise indwi 6-8 (uburemere bw’umubiri 18-22 g) zaguzwe mu kigo ca Beijing Wetahe Laboratory Animal Technology Co., Ltd. amazi mu ndwi imwe kugira ngo zimenyere ibidukikije bishasha.Izo mbeba zaciye zigabanywamwo imigwi ine: umugwi w’abagenzuzi, umugwi w’akarorero ka DSS, umugwi wavuwe na SASP (200 mg/kg, mu kanwa) n’umugwi wavuwe na BLG (1 g/kg, mu kanwa). imbeba n’ingendo zitatu za 1,8% DSS mu misi 5, zikurikirwa n’amazi ayunguruwe mu misi 7, nk’uko bigaragara mu cigwa cacu c’imbere.18 Imbeba zo mu migwi yavuwe na SASP na BLG zavuwe na SASP na BLG, hakurikijwe, buri musi guhera ku musi wa 0.Dukurikije ivyageragejwe vya doBLG vyari vya preliminase. g/kg.Muri ico gihe, urugero rwa SASP rwashizwe kuri 200 mg/Kg hakurikijwe ibitabu.4 Imigwi y’abagenzuzi n’iy’akarorero ya DSS yaronse amazi angana mu gihe cose c’igerageza.
Igishushanyo 1 BLG itera imbere indwara y’umutima isubira inyuma iterwa na DSS mu mbeba.(A) Igerageza ry’ugutegura indwara y’umutima isubira inyuma n’ubuvuzi, (B) uguhinduka kw’uburemere bw’umubiri, (C) igiharuro c’ibikorwa vy’ingwara (DAI), (D) uburebure bw’umubiri, (E) ishusho iserukira umubiri&E×ma (H0) (G) igiharuro c’imitsi.Amakuru yerekanwa nk’agaciro ± SEM (n = 6).##p <0.01 canke ###p <0.001 n’umugwi w’abagenzuzi (Con); *p < 0,05 canke **p < 0,01 canke ***p < 0,001 n’umugwi wa DSS.
Uburemere bw’umubiri, ukuntu amavyi agenda, n’amaraso ava mu nzira y’amaraso vyandikwa buri musi.Ikigereranyo c’ibikorwa vy’ingwara (DAI) camenyekanye mu gufatanya amanota y’uburemere bw’umubiri, ukuntu amavyi agenda neza, n’amaraso ava mu nzira y’amaraso nk’uko vyavuzwe imbere.19 Ku mpera y’igerageza, izindi mbeba zose zaragandaguwe maze amaraso, amavyi arajanwa.
Ivyuma vy’amaraso vyari bishizwe muri formaline maze bishirwa muri parafine.Ibice vy’imikoro 5 vyakozwe maze bishirwako ibara ry’amaraso ryitwa hematoxylin-eosin (H&E), hanyuma birahumwa maze bishirwako amanota nk’uko vyavuzwe imbere.19
ARN yose y’imitsi y’amaraso yavanywe n’ikintu citwa Trizol (Invitrogen, Carlsbad, CA), hanyuma hakurikirwa gukurwamwo cDNA hakoreshejwe ubuhinga bwa reverse transcriptase (TaKaRa, Kusatsu, Shiga, Japan). vyasuzumwe hakoreshejwe uburyo bwa 2-ΔΔCT. Urutonde rw'ivyaduka rwerekanwa mu ndome ya 1.
Ivyiyumviro vy’ugutandukanya utugingo ngengabuzima tw’imbeba n’ugutera imbere vyakozwe nk’uko vyavuzwe mbere.20 Muri make, utugingo ngengabuzima tw’imbeba z’indwi 6-8 twabanje gukurwako ikimazi hakoreshejwe ugusenyuka kw’intanga, hanyuma turafungurwa mu buryo burebure, tuvuzwa n’umuti w’umunyu w’imbeba (HBcium) n’uwutagira. mm y’ibice bitobito.Hanyuma, utugingo ngengabuzima twasimbuwe n’imiti 0,4 mg/mL ya kolagenase XI (Sigma, Poole, UK) mu mazi ya DMEM y’ubuntu hanyuma bica bishirwa mu gitereko c’amazi ku 300 xg mu minota 5 ku bushuhe bwo mu cumba. penicilline, na 100 μg/mL streptomycine) ku 37 °C maze zica mu nzira y’urusenga rw’inylon (ubunini bw’ibihengeri ~250 μm). 5% CO2.
Ivyo bihimba vy’umubiri vyari bihuye n’ivyo bita PBS, kandi urugero rwa cytokines IL-6, TNF-α na IL-1β mu bihimba vy’umubiri vyamenyekanye hakoreshejwe ibikoresho vyo gupima luminex ELISA (R&D systems, Minneapolis, MN, USA). (Bioswamp, Wuhan, mu Bushinwa) nk’uko amabwirizwa y’uwayikoze abivuga.
ADN yose mu mvyimba yavanywe hakoreshejwe igikoresho co gukura ADN (Tiangen, Ubushinwa).Uburyo n’ingero vya ADN vyagereranywa ku rugero rwa 260 nm/280 nm na 260 nm/230 nm, hakurikijwe. 806R (GGACTACHVGGGTWTCTAAT) zakoreshejwe mu gukomeza uturere twa V3-V4 tw’umugera wa 16S rRNA mu turere dutandukanye.Ibintu vya PCR vyasukuwe hakoreshejwe igikoresho co gukuraho ivyuma vy’umubiri (QIAGEN, Ubudage), bigereranywa n’igihe nyaco ca PCR, hanyuma bikurikiranywa hakoreshejwe urubuga rwa Illumina PE3Mi0. CA, USA).Ku bijanye n’isesengura ry’amakuru y’ibinyabuzima, ugutunganya amakuru vyakozwe hakurikijwe amategeko yari yaravuzwe mbere.21,22 Muri make, koresha Cutadapt (V1.9.1) kugira ngo ucungurure amadosiye y’ivy’ubuhinga bwa none. chimeric sequences.Isesengura ry’imiterere y’abanyagihugu n’ugushira mu migwi vyakozwe hakoreshejwe ubuhinga bwo gushikiriza mu migwi RDP (http://rdp.cme.msu.edu/) bushingiye ku rutonde rw’imiterere y’imitsi ya ARN y’imitsi ya SILVA.
Ingero z’amavuta y’uruzitiro rugufi (aside acetique, aside propionique, na aside butyrique) zagereranywa nk’uko vyavuzwe mbere na Tao n’abandi, hariho n’ibindi vyahinduwe.23 Muri make, 100 mg y’amavyi yabanje guhagarikwa mu mazi 0,4 mL. 2-éthylbutyrique acide (ikigereranyo co mu mutima), hanyuma igahindurwa kimwe maze igashushwa ku bushuhe bwa 4°C. 12.000 rpm mu minota 15 ku C.Ivyo bihimba vy’umubiri vyakuwemwo 0,5 mL ya ether hanyuma bishirwa muri GC kugira ngo bisuzumwe. (FID).Ugutandukanya kwashitsweko hakoreshejwe inkingi ya ZKAT-624, 30 m × 0,53 mm × 0,3 μm (Ishirahamwe ry’ubuhinga bwo gusesangura ry’i Lanzhou Zhongke Antai, Ubushinwa). flow rate was 6 mL/min.Igipimo c’urushinge cari 1 μL.Ubushuhe bw’urushinge n’ubw’igipimo bwari 300°C.Ubushuhe bw’ifuru bwagumye kuri 140°C iminota 13,5, hanyuma bugashika kuri 250°C ku rugero rwa 120°C/min; ubushuhe bwagumye iminota 5.
Amakuru yerekanwa nk’agaciro ± ikosa ry’agaciro (SEM).Igisobanuro c’amakuru casuzumwe hakoreshejwe uburyo bumwe bwa ANOVA hakurikijwe ikigeragezo ca Duncan.
Birazwi neza ko UC ari indwara y’ibihaha isubira inyuma idakira ikaba itera ububabare bukomeye bwo mu nda, gucibwamwo n’ugusohoka amaraso. uburemere bw’umubiri bugabanuka kandi DAI igabanywa cane, kandi ayo mahinduka yarahindutse cane inyuma y’imisi 24 umuntu avuwe na BLG (Ishusho 1B na C). histopathological analysis yakozwe kugira ngo hasuzumwe umururumba w’amaraso.Amashusho ya H&E n’ibiharuro vy’indwara vyerekanye ko gufata DSS vyahungabanije cane ubwubatsi bw’amaraso kandi bikaba vyatumye habaho ugusenyuka kw’amaraso, mu gihe ubuvuzi bwa BLG bwagabanyije cane ugusenyuka kw’amaraso n’ingaruka z’uburinzi (Ishusho 1F, the GGse of the pathological scores). g/Kg yari igereranywa n’iya SASP ku rugero rwa 200 mg/Kg.Muri rusangi, ivyo bimenyetso vyerekana ko BLG ari ngirakamaro mu kugabanya uburemere bw’indwara y’ibihaha idakira iterwa na DSS mu mbeba.
TNF-α, IL-1β na IL-6 ni ibimenyetso bihambaye vy’umururumba w’umubiri. Nk’uko vyerekanwa ku gishushanyo 2A, DSS yatumye habaho ukwiyongera gukomeye kw’imiterere y’imitsi ya TNF-α, IL-1β na IL-6 mu mubiri ugereranyije n’umugwi w’abagenzuzi. inflammatory cytokines TNF-α, IL-1β, na IL-6 mu nyama z’umubiri.Ivyo vyavuyemwo vyerekanye kandi ko urugero rwa TNF-α, IL-1β, na IL-6 mu nyama z’umubiri rwariyongereye cane mu mbeba zavuwe na DSS, mu gihe ubuvuzi bwa BLG bwagabanyije ivyo vyiyongereye (Ishusho 2B).
Igishushanyo 2 BLG ibuza ivyerekanwa vy’imitsi n’uguhingura ivyitwa cytokines bitera umururumba TNF-α, IL-1β na IL-6 mu mara y’imbeba zavuwe na DSS.(A) Ivyerekanwa vy’imitsi y’umubiri vyitwa TNF-α, IL-1β na IL-6; (B) urugero rwa poroteyine y’amaraso y’umubiri rwa TNF-α, IL-1β na IL-6.Ivyerekeye amakuru yerekanwa nk’uko bimeze ± SEM (n = 4–6). *p < 0,05 canke **p < 0,01 n’umugwi wa DSS.
Kugira ngo hakorwe itohoza nimba BLG ihindura microbiota y’amara y’imbeba zavuwe na DSS, hakozwe urutonde rwa 16S rRNA kugira ngo hasuzumwe umuryango w’imigera y’ibirimwo mu mara. (Ishusho 3A).Ikindi, urutonde rwa Chao1 n’urutonde rwa Shannon vyerekanwa mu gishushanyo 3B na C vyerekanye ko ubudasa bw’imiryango y’imigera y’amaraso bwagabanutse mu mbeba zavuwe na BLG, kuko urutonde rwa Shannon rwagabanutse cane mu mugwi wavuwe na BLG. patterns hagati y’imigwi itatu kandi yerekanye ko imiterere y’umuryango w’imbeba zavuwe na DSS yari itandukanye neza inyuma y’ukuvugwa na BLG (Ishusho 3D na E).Ivyo bimenyetso vyerekana ko imiterere ya BLG yagira ingaruka zikomeye ku miterere y’umuryango w’imbeba zifise indwara y’ibihaha iterwa na DSS.
Igishushanyo 3 BLG ihindura ubwoko bw’imigera y’amaraso mu mbeba zifise indwara y’ibihaha iterwa na DSS.(A) Igishushanyo ca Venn ca OTU, (B) Index ya Chao1, (C) Index y’ubutunzi bwa Shannon, (D) Isesengura ry’ibice nyamukuru (PCA) igiharuro c’ibiharuro vya OTU, (E) Igiharuro c’ibiharuro vya OTU (Igiharuro ca OTU PCo Coordinate) Amakuru yerekanwa nk’uko ari hagati ± SEM (n = 6).**p <0.01 vs umugwi wa DSS.
Kugira ngo dusuzume amahinduka yihariye mu binyabuzima vyo mu mara, twasuzumye igihimba c’ibinyabuzima vyo mu mara ku rwego rwose rw’ubuhinga. Nk’uko vyerekanwa ku gishushanyo ca 4A, ubwoko nyamukuru mu mirwi yose bwari Firmicutes na Bacteroidetes, bukurikirwa na Verrucomicrobia. imiryango y’imbeba zavuwe na DSS ugereranyije n’imbeba zigenzurwa, kandi ayo mahinduka yarahindutse cane inyuma y’ukuvugwa na BLG .Cane cane, kuvugwa na BLG kwatumye habaho ubwinshi bw’imigera ya Verrucobacterium mu mara y’imbeba zifise indwara y’ibihaha iterwa na DSS. Muribaculaceae, Akkermansia, Ruminococcaceae na Prevotellaceae (Ishusho 4B).Ugereranije n’umugwi wa DSS, ugutakaza BLG vyatumye ubwinshi bw’ibikoko vy’ibikoko vy’ibikoko vy’ibikoko vy’ibikoko vy’ibikoko vy’ibikoko vy’ibikoko vy’ibikoko vy’ibikoko vy’ibikoko vy’ibikoko vy’ibikoko vy’ibikoko vy’ibikoko vy’ibikoko vy’ibikoko. Ivyo vyavumbuwe kandi vyerekanye ko ubuvuzi bwa BLG bwahinduye neza ubusumbasumbane bw’ibinyabuzima bitobito mu kwishura ku ngorane za DSS, bugaragazwa n’ukugabanya Eubaxynogroup_group, Ruminococcaceae_UCG-014, Intestinimonas na Oscillibacter, n'ukwiyongera kw'ibikoko vy'ibikoko vy'ibikoko vy'ibikoko vy'ibikoko vy'ibikoko vy'ibikoko vy'ibikoko vy'ibikoko vy'ibikoko vy'ibikoko.
Igishushanyo 4 BLG ihindura ubwinshi bw’imigera yo mu mara mu mbeba ziterwa na DSS.(A) Ubwinshi bw’imigera yo mu mara ku rugero rwa phylum; (B) Ubwinshi bw’imigera yo mu mara ku rugero rw’umuryango; (C) Ubwinshi bw’imigera yo mu mara ku rugero rw’ubwoko.Amakuru yerekanwa nk’agaciro ± SEM (n = 6).#p < 0.05 canke ###p < 0.001 vs umugwi w’abagenzuzi (Con); *p < 0,05 canke **p < 0,01 canke ***p < 0,001 n’umugwi wa DSS.
Turavye ko aside z’ibinure zifise uruzitiro rugufi (SCFAs) ari zo zihindura cane ibinure vy’ibimera vy’ibimera vy’ibimera vy’ibimera vy’ibimera vy’ibimera vy’ibimera vy’ibimera vy’ibimera vy’ibimera vy’ibimera, mu gihe acetate, propionate na butyrate ari vyo bihindura ibinure vyinshi cane mu nzira y’amaraso, 25-27 turacari mu nyigisho yacu.Nk’uko vyerekanwa ku gishushanyo, 5. ryagabanutse cane mu mugwi wavuwe na DSS, mu gihe ubuvuzi bwa BLG bwoshobora guhagarika cane iyo nzira.
Igishushanyo 5. BLG yongera urugero rwa SCFAs mu mvyimba z’imbeba zifise indwara y’ibihaha iterwa na DSS.(A) Aside acetique iri mu mvyimba; (B) aside propionique iri mu mva; (C) aside butyrique irimwo mu mara.Amakuru yerekanwa nk’agaciro ± SEM (n = 6).#p < 0.05 canke ##p < 0.01 vs umugwi w’abagenzuzi (Con); *p < 0,05 canke **p < 0,01 n’umugwi wa DSS.
Twarabaruye kandi igiharuro c’isano hagati ya SCFA itandukanye ku rugero rw’ubwoko n’imigera y’amavyi. Nk’uko vyerekanwa ku gishushanyo ca 6, Akkermansia yari ifitaniye isano ryiza n’uguhingura aside propionique (Pearson = 0.4866) n’aside butyrique (Pearson = 0.6192 Oscimonac yari ifitaniye isano ribi na Enterolloc bo). ivyimburwa vy’acetate, n’ibiharuro vya Pearson vy’ibiharuro 0,4709 na 0,5104, hakurikijwe.Na vyo nyene, Ruminococcaceae_UCG-014 vyari bifitaniye isano ribi n’ivyimburwa vy’aside ya propiyonike (Pearson = 0,4508) n’ivy’aside ya butiriki (Pearson = 0,2).
Igishushanyo 6 Isesengura ry’isano hagati ya SCFAs zitandukanye n’imigera y’amaraso.(A) Enteromonas n’aside acetique; (B) Bacillus y’uguhungabana kw’umubiri n’aside acetique; (C) Akkermansia n’aside ya propiyonike; (D) Igikoko c’umugera_UCG-014 gifise aside ya propiyonike; (E) Igikoko c’umukara gifise aside ya butirike; (F) ) Igitera _UCG-014 gifise aside ya butirike.
Nk’uko vyerekanwa ku gishushanyo ca 7, DSS yatumye habaho ukugabanuka gukomeye kw’imvugo ya Gcg mRNA. (Ishusho 7A).Muri ico gihe nyene, urugero rwa GLP-1 mu maraso rwagabanutse cane mu mugwi wavuwe na DSS, kandi ubuvuzi bwa BLG bwari gushobora cane gukingira iyo nzira (Ishusho 7B). 41 (GRP41) activation, twasuzumye kandi GPR41 na GRP43 mu mara y’imbeba zifise indwara ya colitis maze tubona ko mRNA y’amaraso ya GRP43 na GPR41 yagabanutse cane inyuma y’ingorane ya DSS, kandi ubuvuzi bwa BLG bwashobora gukiza neza ivyo bigabanutse na DC (Ishusho 7).
Igishushanyo 7 BLG yongera urugero rwa GLP-1 mu maraso n’uguserura Gcg, GPR41 na GRP43 mRNA mu maraso mu mbeba zavuwe na DSS.(A) Guserura mRNA ya Gcg mu maraso; (B) Urugero rwa GLP-1 mu maraso; (C) GPR41 mRNA igaragara mu nyama z’umubiri; (D) GPR43 mRNA igaragara mu nyama z’umubiri.Amakuru yerekanwa nk’agaciro ± SEM (n = 5–6).#p <0.05 canke ##p <0.01 vs umugwi w’abagenzuzi (Con); *p < 0,05 n’umugwi wa DSS.
Kubera ko ubuvuzi bwa BLG bushobora kwongerera urugero rwa GLP-1 mu maraso, ivyerekanwa vya Gcg mRNA mu maraso, n’urugero rwa SCFA mu maraso mu mbeba zavuwe na DSS, twasuzumye kandi ivyerekeye acetate, propionate, na butyrate be n’ivyo bivuye ku kugenzura (F-Con), DSS colitis (F-Con) -DSS) na coBLGtisce the BLG- GLP-1 iva mu turemangingo tw’umubiri w’imbeba.Nk’uko vyerekanwa ku gishushanyo 8A, utugingo ngengabuzima tw’imbeba twavuwe n’aside acetique 2 mM, aside propionique, n’aside butyrique, hakurikijwe, twatumye GLP-1 irekurwa cane, bihuye n’ivyigwa vya kera.29, FBLSS-30 Nk’uko nyene, FBLSSCon (bingana na 0,25 g y’amavyi) vyatumye cane GLP-1 isohoka mu turemangingo tw’umubiri w’imbeba. Biragaragara ko umubare wa GLP-1 urekurwa n’uturemangingo tw’imbeba twavuwe na F-DSS wari muto cane kuruta uw’uturemangingo tw’imbeba twavuwe na F-DSS twavuwe na F-DSS wari muto cane kuruta uw’uturemangingo tw’imbeba twavuwe na F-DSS twavuwe na F-DSS twavuwe na F-DSS. 8B).Ivyo bimenyetso vyerekana ko ubuvuzi bwa BLG bwagaruye cane ubushobozi bwo gukora GLP-1 ikomoka kuri SCFA mu mara.
Igishushanyo 8 SCFA ikomoka kuri BLG itera GLP-1 kurekura mu turemangingo tw’imbere tw’umubiri w’inyamaswa.(A) Asidi asetike, aside propionique, na aside butyrique vyatuma GLP-1 irekurwa mu ngingo z’umubiri z’intango z’umubiri w’inyamaswa; (B) ivyimburwa vy’amavyi F-Con, F-DSS na F-BLG vyavyuriye utugingo ngengabuzima tw’umubiri w’inyamaswa z’ingurube Igitigiri ca GLP-1 yasohotse. F-BLG (ingana na 0,25 g y’amavyi), uko bigenda. Amasaha 2 ku 37°C, 5% CO2, hakurikijwe.Igitigiri ca GLP-1 kiva mu turemangingo tw’umubiri w’inyamaswa z’ingurube za mbere zabonetse biciye ku nzira ya ELISA. *p < 0,05 n’ivyo F-DSS.
Inyuguti zigufi: Ace, aside asetike; Pro, aside ya propiyonike; ariko rero, aside butirike; F-Con, amavyi ava mu mbeba zigenzurwa; F-DSS, amavyi ava mu mbeba zifise indwara ya colite; F-BLG, ivuye mu maraso yavuwe na BLG Amavyi y’imbeba zifise umururumba.
UC iri ku rutonde rw’indwara zidashobora gukira n’ishirahamwe mpuzamakungu ryitaho amagara y’abantu, iriko iraba akaga kw’isi yose; ariko rero, uburyo bwiza bwo kumenya imbere y’igihe, gukingira no kuvura iyo ndwara buracari buke. Ku bw’ivyo, hariho ivyihutirwa vyo gutohoza no gutegura uburyo bushasha bwo kuvura UC. kandi vyose ni ibinyabuzima n’ibikoresho vy’ibinyabuzima ahanini bitagira akamaro ku bantu n’ibikoko.31,32 Iyi nyigisho yari igamije kurondera umuti w’imiti y’abashinwa utekanye kandi ukora neza wo kuvura UC no kumenya uburyo ukora.BLG ni umuti uzwi cane w’ibimera vy’abashinwa ukoreshwa mu kuvura ibicurane mu bikorwa vyacu3. indigo, umuti w’imiti y’abashinwa ukozwe mu bikoresho bimwe na BLG, ugaragaza ubushobozi buhambaye mu kuvura UC ku bantu no ku bikoko.4,34 Ariko rero, ingaruka z’ubuvuzi bwa BLG n’ingaruka zayo Uburyo bukoreshwa ntibutomoye. umururumba, ukaba ufitaniye isano n’uguhindura imigera y’amaraso no gusubizaho ivyimburwa vya GLP-1 biva mu mara.
Birazwi neza ko UC irangwa n’ibihe vy’ugusubira mu ndwara bifise ibimenyetso vy’ubuvuzi bimenyerewe, nk’ugutakaza ibiro, guciramwo, kuva amaraso mu nzira, no kwonona cane umubiri w’umubiri.35 Gutyo, indwara y’umutima isubira inyuma idakira yatanzwe mu gutanga inzinguzingu zitatu za dverinking 1,8% da DSS ku myaka 1,8. amazi.Nk’uko vyerekanwa ku gishushanyo 1B, uguhinduka kw’uburemere n’ibiharuro vya DAI vyerekana ko umuntu yashoboye gutera neza indwara y’ibihaha idakira. Imbeba zo mu mugwi wavuwe na BLG zerekanye ko zakize kuva ku musi wa 8, ivyo bikaba vyari bitandukanye cane n’ivyo ku musi wa 24. colitis.Ku bijanye n’ugukomeretswa kw’amaraso n’imimerere y’umururumba, uburebure bw’amaraso, ukwononekara kw’amaraso, n’uguserura n’uguhingura ivyitwa cytokines bitera umururumba TNF-α, IL-1β, na IL-6 mu mara na vyo nyene vyarateye imbere cane inyuma y’ubuvuzi bwa BLG. imbeba.
Ni gute BLG ikoresha ingaruka zayo ku miti?Ivyigwa vyinshi vya kera vyerekanye ko imigera yo mu mara igira uruhara runini mu gutera indwara ya UC, kandi ubuvuzi bushingiye ku migera n’ubuvuzi bushingiye ku migera bwaserutse nk’ubuhinga bukwegera cane bwo kuvura UC. ihinduka mu mibumbe y’imigera y’amaraso, bikaba vyerekana ko ingaruka z’uburinzi za BLG ku ndwara y’umusonga iterwa na DSS zifitaniye isano n’uguhindura imigera y’amaraso.Ivyo vyiyumviro bihuye n’iciyumviro c’uko gusubira gutegura imiterere y’imigera y’amaraso ari uburyo buhambaye bwo gutahura ubushobozi bw’imiti ya TCM Gramantaker-a76.36,3. strictly anaerobic bacterium iba mu gice c’umubiri c’umubiri, iyo na yo igasenya umubiri w’umubiri, igatuma habaho aside propionique, igatuma utugingo ngengabuzima tw’umubiri dutandukana, kandi igatuma umubiri ugumaho. Amakuru menshi yerekeye ubuvuzi n’ibikoko yerekana ko Akkermansia ifitaniye isano cane n’umubiri ufise amagara meza,38 be n’ugufata Akkermansia spp. birashobora gutuma umururumba w’umubiri utera imbere cane.39 Amakuru dufise ubu yerekana ko ubwinshi bw’ibikoko vya Akkermansia bugenda burushirizaho inyuma yo kuvugwa na BLG.Ikindi, Prevotellaceae_UCG-001 ni umugera utanga SCFA.27 Ivyigwa vyinshi vyerekanye ko ubwinshi bw’ibikoko vy’ibikoko vy’ibikoko vy’ibikoko vy’ibikoko vy’ibikoko vy’ibikoko vy’ibikoko vy’ibikoko vy’ibikoko vy’ibikoko vy’ibikoko. 40,41 Amakuru yacu yerekana kandi ko ubuvuzi bwa BLG bushobora kwongerera cane ubwinshi bwa Prevotellaceae_UCG-001 mu mara y’imbeba zavuwe na DSS. UC imbeba kandi yari ifise isano ryiza cane n’ingero za IL-6 na IL-1β be n’ibiharuro vy’indwara.43,44 Biragaragara ko ubuvuzi bwa BLG bwagabanuye cane ubwinshi bwa Oscillibacter mu mara y’imbeba zavuwe na DSS. 45,46 Amakuru yacu y’ubu yerekanye kandi ko ivyiyumviro vya SCFA acetate, propionate, na butyrate mu mavyi yavuwe na DSS vyari vyiyongereye cane mu mbeba zavuwe na BLG bishobora kwerekana neza ko ivyo bimenyetso bishobora gufasha. n’ugutuma imigera itera SCFA iterwa na DSS itera imbere mu mbeba zifise indwara y’ibihaha idakira.
GLP-1 ni incretin ivugwa cane cane mu gifu no mu gifu kandi ifise uruhara runini mu gutevya gusohoka kw’inda no kugabanya isukari mu maraso inyuma y’ifunguro.47 Ivyemezo vyerekana ko dipeptidyl peptidase (DPP)-4, umuti ugabanya ububabare bw’umubiri, n’umuti w’ubumara bwa GLP-1 ushobora gufasha neza 1. vyavuzwe mu nyigisho za kera, SCFA nyinshi zari zifitaniye isano n’urugero rwa GLP-1 mu maraso y’abantu no mu mbeba. 52 Amakuru yacu yerekana ko inyuma y’ukuvugwa na BLG, urugero rwa GLP-1 mu maraso n’uguseruka kwa Gcg mRNA vyariyongereye cane. Na vyo nyene, ivyimburwa vya GLP-1 vyariyongereye cane mu mico kama y’amakoloni inyuma yo gukangura n’ibiva mu mavyi y’imbeba zavuwe na BLG zifise indwara ya colitis ugereranyije n’ugukangura n’ibiva mu mavyi y’imbeba zavuwe na BLG zifise indwara ya colitis. bigira ico bikoze ku kurekura GLP-1?Gwen Tolhurst n’abandi. SCFA ishobora gutuma GLP-1 isohoka biciye ku GRP43 na GPR41.29 Amakuru dufise ubu yerekana kandi ko ubuvuzi bwa BLG bwongerera cane mRNA ya GRP43 na GPR41 mu maraso y’imbeba zavuwe na DSS.
BLG ni umuti ukoreshwa mu gihe kirekire mu Bushinwa. Igitigiri c’imiti BLG ishobora kwihanganirwa mu mbeba zo muri Kunming ni 80g/Kg, kandi nta bumara bukomeye bwabonetse.53 Ubu, igitigiri c’imiti BLG (itagira isukari) mu bantu ni 9-15 g/umunsi (incuro 3 ku musi).Icigwa cacu cerekanye ko ameliinduated BLSS/K Iyi doze iri hafi y’iyo doze ya BLG ikoreshwa mu buvuzi.Icigwa cacu kandi casanze uburyo ikora bufashwa, n’imiburiburi mu gice, n’uguhinduka kw’ibinyabuzima vyo mu mara, cane cane imigera itera SCFA mu mara, nka Akkermansia na Prevotella-rest_LP-re1 to01. Ivyo bimenyetso vyerekana ko BLG ikwiye gusubirwamwo nk’umuti wo kuvura indwara y’ibihaha. Ariko rero, uburyo nyabwo ikoresha mu guhindura imigera y’amaraso buracariho kugira ngo yemezwe n’imbeba zidafise imigera y’amaraso be n’ugutera imigera y’amavyi.
Ace, aside ya asetike; ariko, aside butirike; BLG, igiti c’umukaraba; DSS, ni ukuvuga umuti w’ivyatsi bita dextran; DAI, urutonde rw’ibikorwa vy’ingwara; DPP, ni ukuvuga umusemburo w’ibinyabuzima; FID, igikoresho co kumenya iyonizasiyo y’urubeya; F-Con, kugenzura Ivyimburwa vy’imbeba; F-DSS, amavyi y’imbeba zifise indwara ya DSS; F-BLG, ivyimburwa vy’amavyi y’imbeba zavuwe na BLG; GLP-1, ni umugera umeze nk’uw’isukari-1; Gcg, isukari; ivy’ugupima ibara ry’umuyagankuba, ivy’ugupima ibara ry’umuyagankuba; GRP43, igihimba c’umubiri gifatanye n’ivyo bita G 43; GRP41, igihimba c’umubiri gifatanye n’ivyo bita G 41; H&E, ni ukuvuga amaraso y’umubiri; HBSS, Umuti w’Umunyu Uringaniye wa Hanks ; OTC, OTC; PCA, isesengura ry’ibice nyamukuru; PCoA, isesengura ry’imigenderanire nyamukuru; Pro, aside ya propiyonike; SASP, umuti wo gutera ivyatsi; SCFA, ni aside zifise urudodo rugufi; Ubuvuzi bw’abashinwa, ubuvuzi bwa kera bw’abashinwa; UC, indwara y’ibisebe.
Amategeko yose y’igerageza yemejwe na Komite y’inyifato runtu y’ibikoko yo muri kaminuza ya Peking Shenzhen-Ikigo c’ubuvuzi ca kaminuza ya Hong Kong y’ubuhinga n’ubuhinga (Shenzhen, Ubushinwa) hakurikijwe amabwirizwa y’inzego n’amabwirizwa agenga ibikoko (inyifato runtu inomero A2020157).
Abanditsi bose baragize uruhara runini mu gutegura no guhingura, mu kuronka amakuru, canke mu gusesangura no kuyasobanura; yaragize uruhara mu gutegura iyo ngingo canke mu gusubiramwo mu buryo bubereye ibintu bihambaye vyo mu bwenge; yemeye gutanga iyo nyandiko y’ikiganza mu kinyamakuru kiriho ubu; amaherezo yemeje ko iyo verisiyo isohoka; Ujejwe imice yose y’igikorwa.
Ico gikorwa cashigikiwe n’ishirahamwe ry’igihugu ry’ubuhinga bwa kavukire ryo mu Bushinwa (81560676 na 81660479), umugambi wo mu rwego rwa mbere wa kaminuza ya Shenzhen (86000000210), ikigega ca komite y’ubuhinga n’ubuhinga bushasha y’i Shenzhen (JCYJ202103240938100), n’ubuhinga bwa none. Ikigega c’ubushakashatsi (A2020157 na A2020272), Ivuriro ry’ubuvuzi rya kaminuza ya Guizhou Intara ya Guizhou ryaterwa inkunga n’ikigo c’ubushakashatsi (YWZJ2020-01) n’ibitaro vya kaminuza ya Peking (JCYJ2018009).
1. Tang B, Zhu J, Zhang B, n'abandi.
2. Kaplan GG.Umuzigo w’isi yose w’indwara y’umutima: kuva mu 2015 gushika mu 2025.
3. Peng J, Zheng TT, Li Xue, n’abandi.
4. Xiao Haiteng, Peng Jie, Wen B, n'abandi.
5. Chen M, Ding Y, Tong Z. Ubushobozi n’umutekano w’imiti y’ibimera y’Abashinwa mu kuvura indwara y’ibisebe: ibimenyamenya vy’ubuvuzi n’ubushobozi uburyo.Imbere y'imiti.2020;11:603476.doi:10.3389/ffhar.2020.603476
6. Cao Fang, Liu Jie, Sha Benxing, Pan HF.Ibintu biva mu kamere: imiti ivura indwara y’umururumba w’amara.
7. Zhang C, Jiang M, Lu A. Ivyiyumviro ku buvuzi bw’inyongera bw’indwara y’ibisebe vy’umutima n’ubuvuzi bwa kera bw’Abashinwa.
8. Li Zhongteng, Li Li, Chen TT, n’abandi.